The flow cytometer Diaries

The flow cytometer Diaries

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Knowledge-enriched characterization of cell populations. Morphological info from illustrations or photos can include into the richness of flow cytometry knowledge. As an example, the determine displays an if not standard apoptosis assay working with Annexin V and PI, incorporating cell imaging to characterize cells in Each and every inhabitants to expose morphologically distinctive capabilities. These insights couldn't happen to be gained from multiplex staining by itself.

No matter whether you’re a novice or an expert, investigate our sources to advance your investigate and expertise in flow cytometry.

Incorporate acoustic focusing technology with higher-parameter Investigation for versatile spectral unmixing and standard workflows.

The award-profitable Invitrogen Attune CytPix Flow Cytometer combines acoustic concentrating having a higher-velocity brightfield camera for simultaneous substantial throughput flow cytometry and substantial resolution brightfield imaging.

"An image isn't fairly really worth 1000 dots, but it truly helps to see visuals. It is excellent to discover pictures that correlate with staining. One example is, AnnexinV-optimistic cells which might be morphologically different than wholesome cells, or CD14+ monocytes that happen to be more substantial and much more textured than CD3+/CD4+/CD14- lymphocytes.

Moreover, it is actually illuminating to check out the amount of doublets slip through standard singlet gates, and terrific to verify the status of sudden double-constructive events in my analysis."

Optimizing gating strategy. Even sturdy manual singlet gating is mistake-vulnerable and remains a subjective choice position in almost all flow cytometry assays. Imaging can be used to confirm and regulate gates to include only one cells of interest.

Our scientists will layout panels for you as persistently you'll need—cost-free, no item invest in essential.

The Attune NxT cytometer will be the reliable workhorse instrument your lab can trust in for steady and dependable overall performance. The Attune CytPix model encompasses a significant-speed brightfield camera and AI-pushed graphic analysis application, with modern updates which include machine Studying instruments and an intuitive user interface, enabling enhanced knowledge precision, precision, and a lot quicker insights into sample biology.

Perhaps most significantly, these events include cells of Plainly distinctive phenotypes which could lead to incorrect conclusions pertaining to double favourable gatherings (particularly in scarce populations).

Sound waves permit specific cell alignment, causing Extraordinary data high quality and ~10X faster sample flow charges.

Application Notice: Automatic graphic analysis reduces consumer-to-consumer variability in flow cytometry gating tactics

Leverage the strength of Sophisticated technology and innovation to speed up your discoveries. Belief while in the Attune Flow Cytometer household to provide the reliability, speed, and precision that set your do the job aside.

No wash/no lyse leukocyte Investigation with imaging. Cells had been acquired from 24-hour-outdated blood by dilution in 1 mM EDTA ( Professional UX/UI allows people to practice AI models for expanded programs. (A) The “Train Design” characteristic supplies annotation tools and serious-time design instruction precision.

Gating on these extended parameters lets you rapidly and precisely establish populations of interest and make sure gating technique with little if any manual review. The Attune program presents access to state-of-the-art equipment Discovering Examination tools inside of an expertly developed consumer interface, making it exceptionally click here convenient to use.

The Attune Xenith cytometer is often a large-parameter spectral analyzer, offering both spectral unmixing and standard flow abilities for thorough and flexible cell Assessment.

The Attune CytPix Flow Cytometer allows customers to visualize samples all through acquisition, confirm the accuracy of gating procedures, and aid final results with trusted documentation for publication reasons.

What appeared to be a homogeneous population is actually much more assorted—an Perception that ought to be regarded as when interpreting final results.